Direct electron transfer of glucose oxidase immobilized in an ionic liquid reconstituted cellulose-carbon nanotube matrix

Abstract

A conductive cellulose – multiwalled carbon nanotube (MWCNT) matrix with a porous structure and good biocompatibility has been prepared by using room temperature ionic liquids (1–ethyl–3–methylimidazolium acetate) as solvent. Glucose oxidase (GOx) was encapsulated in this matrix and thereby immobilized on surface of a glassy carbon. The direct electron transfer and electrocatalysis of the encapsulated GOx has been investigated using cyclic voltammetry and chronoamperometry. The GOx exhibited a pair of stable, well defined and nearly symmetric reversible redox peaks. The experimental results also demonstrate that the immobilized GOx retains its biocatalytic activity toward the oxidation of glucose, which can be employed to determine the glucose concentration. The results showed that the bioelectrode modified by the reconstitute cellulose–MWCNT matrix has great potential for use as a biosensor and other bioelectronics devices.

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